Search
- Page Path
- HOME > Search
- Characterization of Antimicrobial Susceptibility, Extended-Spectrum β-Lactamase Genes and Phylogenetic Groups of Enteropathogenic
Escherichia coli Isolated from Patients with Diarrhea - Erfaneh Jafari, Saeid Mostaan, Saeid Bouzari
- Osong Public Health Res Perspect. 2020;11(5):327-333. Published online October 22, 2020
- DOI: https://doi.org/10.24171/j.phrp.2020.11.5.09
- 7,754 View
- 104 Download
- 5 Web of Science
- 7 Crossref
-
Abstract
PDF Objectives Infectious diarrhea is one of the most common causes of pediatric death worldwide and enteropathogenic
Escherichia coli (EPEC) is one of the main causes. There are 2 subgroups of EPEC, typical and atypical, based on the presence or absence of bundle forming pili (bfp), of which atypical EPEC is considered less virulent, but not less pathogenic. Antimicrobial resistance towards atypical EPEC among children is growing and is considered a major problem. In this study the pattern of antibiotic resistance in clinical isolates was determined.Methods Using 130 isolates, antibiotic resistance patterns and phenotypes were assessed, and genotypic profiles of extended spectrum β-lactamase (ESBL) production using disc diffusion and PCR was carried out. Phylogenetic groups were analyzed using quadruplex PCR.
Results There were 65
E. coli isolates identified as atypical EPEC by PCR, among which the highest antibiotic resistance was towards ampicillin, followed by trimethoprim-sulfamethoxazole, and tetracycline. Multidrug resistance was detected in 44.6% of atypical EPEC isolates. Around 33% of isolates were determined to be extended spectrum β-lactamase producers, and in 90% of isolates, genes responsible for ESBL production could be detected. Moreover, the majority of atypical EPEC strains belonged to Group E, followed by Groups B1, B2 and C.Conclusion High rates of multidrug resistance and ESBL production among atypical EPEC isolates warrant periodical surveillance studies to select effective antibiotic treatment for patients. It is considered a critical step to manage antibiotic resistance by avoiding unnecessary prescriptions for antibiotics.
-
Citations
Citations to this article as recorded by
- Phenotypic and molecular characterization of enteropathogenic Escherichia coli and Salmonella spp. causing childhood diarrhoea in Awka, South-Eastern Nigeria
Ifeanyi Emmanuel Nwike, Malachy Chigozie Ugwu, Peter Chika Ejikeugwu, Nonye Treasure Ujam, Ifeanyichukwu Romanus Iroha, Charles Okechukwu Esimone
Bulletin of the National Research Centre.2023;[Epub] CrossRef - Antimicrobial Resistance and Biofilm Formation of Escherichia coli Isolated from Pig Farms and Surroundings in Bulgaria
Mila D. Kaleva, Yana Ilieva, Maya Margaritova Zaharieva, Lyudmila Dimitrova, Tanya Chan Kim, Iva Tsvetkova, Yordan Georgiev, Petya Orozova, Krasimir Nedev, Hristo Najdenski
Microorganisms.2023; 11(8): 1909. CrossRef - Isolation and Molecular Characterization of Proteus mirabilis Bacteria from Poultry Meat in the Iraqi Market and a Study of their Resistance to some Antibiotics
Ammar H. Hamed, Ahmed I. Alnazzal
IOP Conference Series: Earth and Environmental Sci.2023; 1262(6): 062019. CrossRef - Virulence and phylogenetic analysis of enteric pathogenic Escherichia coli isolated from children with diarrhoea in South Africa
Ntando W. Alfinete, John Y. Bolukaoto, Lee Heine, Natasha Potgieter, Tobias G. Barnard
International Journal of Infectious Diseases.2022; 114: 226. CrossRef - Prevalence of extended-spectrum β-lactamases, AmpC, and carbapenemases in Proteus mirabilis clinical isolates
Mona Shaaban, Soha Lotfy Elshaer, Ola A. Abd El-Rahman
BMC Microbiology.2022;[Epub] CrossRef - Enteropathogenic Escherichia coli—A Summary of the Literature
Anca Delia Mare, Cristina Nicoleta Ciurea, Adrian Man, Bianca Tudor, Valeriu Moldovan, Luminița Decean, Felicia Toma
Gastroenterology Insights.2021; 12(1): 28. CrossRef - Prevalence of Antibiotic-Resistant Escherichia coli Isolated from Swine Faeces and Lagoons in Bulgaria
Lyudmila Dimitrova, Mila Kaleva, Maya M. Zaharieva, Christina Stoykova, Iva Tsvetkova, Maya Angelovska, Yana Ilieva, Vesselin Kussovski, Sevda Naydenska, Hristo Najdenski
Antibiotics.2021; 10(8): 940. CrossRef
- Phenotypic and molecular characterization of enteropathogenic Escherichia coli and Salmonella spp. causing childhood diarrhoea in Awka, South-Eastern Nigeria
First
Prev
